Goals of the NanoTENDO
WP number: 1
WP title: Nanoparticles preparation (synthesis)
Leader: P2 – UAH
Partners involved: P2 – UAH
Start date: 1
End date: 32
Objectives: Synthesis and physico-chemical characterization of nanoparticles
Task 1. Synthesis of novel carbosilane-based nanomaterials, including dendrimers, dendrons and gold NPs. Heterofunctionalization with traceable, solubilizing, targeting moieties and drugs.
Task 2. Physico-chemical characterization of NPs by several techniques
WP number: 2
WP title: Biophysical characterization of nanoparticle/drug complexes.
Leader: P1 – UL
Partners involved: P1 – UL
Start date: 4
End date: 24
Objectives: Evaluation of biological activity (toxicity and genotoxicity) of the synthesized nanosystems
Task 1. Preliminary fast screening of biological properties of nanomaterials on the basis of analysis of their toxicity in in vitro tests (genotoxicity, haemotoxicity, cytotoxicity by MTT test/ LDH assay, microscopy).
Task 2. Task 2. Studying of forming complexes between nanoparticles and drugs and immunomodulatory compounds (short dsDNA and ssRNA) by a wide range of biophysical techniques (electrophoresis, fluorescence methods, zeta potential measurements, microscopy)
Task 3. Characterization of complexes: toxicity (haemotoxicity, cytotoxicity by MTT test/ LDH assay, microscopy), morphology (TEM, AFM), zeta potential (Laser Doppler Electrophoresis), hydrodynamic size (Dynamic Light Scattering), stability in different conditions: stability in time of incubation, stability in presence of nucleases, proteases, heparin, serum proteins (electrophoresis, fluorescence).
WP number: 3
WP title: Efflux of NPs and their complexes with drugs through the model endothelial barrier in vitro and in vivo
Leader: P2 – UAH
Partners involved: P1 – UL, P3 – RSU, P4 - LBMC
Start date: 10
End date: 36
Objectives: Synthesis and physico-chemical characterization of nanoparticles
Task 1. To study the uptake, cytotoxicity and efflux of NPs through the model endothelial barrier using molecular and cell biology techniques (flow cytometry, cytotoxicity assays, confocal and atomic force microscopy).
Task 2. To estimate the functional state of the endothelial barrier cells in presence of NPs and their complexes: the state of active/passive transport, cell proliferation and morphology, using by flow cytometry, confocal microscopy, biochemical assays.
Task 3. To perform the tasks (1) and (2) in stress conditions induced by oxidative stress and acidation (this model is related to brain ischemia), and in presence of amyloid-beta peptides (related to Alzheimer disease).
Task 4. To test capacity of selected NPs to transpass EB and BBB in vivo.
WP number: 4
WP title: Testing of dendrimers and dendronized NPs for the capacity to reduce inflammation and treat ischemic stroke and Alzheimer disease in a mouse model
Leader: P3 - RSU
Partners involved: P3 - RSU, P4 - LBMC
Start date: 3
End date: 36
Objectives: Establishing of murine models (MM) of ischemic stroke (IS) with the histopathological verification. Evaluation of NP and NP/drug effects on IS and AD pathology in vivo
Task 1. To establish murine models (MM) of ischemic stroke (IS) with histopathological verification (IS)
Task 2. To establish exposure mouse model of AD with histopathological verification.
Task 3. To evaluate the effects of selected complexes of NPs with drugs and immunomodulatory compounds on IS and AD pathology in mouse models.
WP number: 5
WP title: Nanoparticles preparation (synthesis)
Leader: P1 – UL
Partners involved: P1- UL, P2 - UAH, P3 - RSU, P4 - LBMC
Start date: 1
End date: 36
Objectives: Management structure and decision making
Task 1. The common management will be provided by all participants.
Task 2. Activities/events carried out for management of project. The current task
Task 3. Allocation of responsible WP and task leaders
Task 4. Creation and support of the project Web site
Time schedule of the work packages and their components.
