WP number: 2
WP title: Biophysical characterization of nanoparticle/drug complexes.
Leader: P1 – UL
Partners involved: P1 – UL
Start date: 4
End date: 24
Objectives: Evaluation of biological activity (toxicity and genotoxicity) of the synthesized nanosystems
Task 1. Preliminary fast screening of biological properties of nanomaterials on the basis of analysis of their toxicity in in vitro tests (genotoxicity, haemotoxicity, cytotoxicity by MTT test/ LDH assay, microscopy).
Task 2. Task 2. Studying of forming complexes between nanoparticles and drugs and immunomodulatory compounds (short dsDNA and ssRNA) by a wide range of biophysical techniques (electrophoresis, fluorescence methods, zeta potential measurements, microscopy)
Task 3. Characterization of complexes: toxicity (haemotoxicity, cytotoxicity by MTT test/ LDH assay, microscopy), morphology (TEM, AFM), zeta potential (Laser Doppler Electrophoresis), hydrodynamic size (Dynamic Light Scattering), stability in different conditions: stability in time of incubation, stability in presence of nucleases, proteases, heparin, serum proteins (electrophoresis, fluorescence).